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Image Search Results
Journal: Cell reports
Article Title: Single-cell analysis of early chick hypothalamic development reveals that hypothalamic cells are induced from prethalamic-like progenitors
doi: 10.1016/j.celrep.2021.110251
Figure Lengend Snippet: (A–C) UMAP plots showing (A) HH13–HH16 integrated clusters and annotations, (B) distribution of representative genes demarcating hypothalamic progenitor and neurogenic regions, and (C) scRNA-seq mammillary/supramammillary, tuberal, and prethalamic trajectories obtained from RNA velocity. (D) Schematic sideview showing developing hypothalamic regions and adjacent domains; colored regions approximate their counterparts in and . (E–J”) Maximum intensity projections of hemi-dissected HH14–HH15 heads after wholemount in situ HCR triple labeling with combinations of regional progenitor markers. Anterior to the left. (K–M) Pseudotime analysis of mammillary/suprammillary hypothalamus (K), tuberal hypothalamus (L), and prethalamus (M). (N) UMAP plot showing distribution of HH13/14 and HH15/16 cells across clusters. (O–Q) Maximum intensity projections of hemi-dissected HH13–HH15 heads after wholemount in situ HCR to show patterns of neurogenesis and onset of tuberal-specific neural precursor cells. (R and R’) Transverse section through the tuberal hypothalamus and optic stalk after triple in situ HCR for ELAVL4 , NR5A1 , and HCRT (n = 5–8 embryos each for E–I and O–R). Scale bars, 100 μm. Dien, diencephalon; MM, mammillary; NPC, neuronal precursor cells; OM, optic midline; Prog, progenitors; SM, supramammillary; Tub, tuberal. Other abbreviations as per and .
Article Snippet:
Techniques: In Situ, Labeling